PDCD1 and cancer: Correlations between ANS signatures and levels of selected molecular markers of angiogenesis, hypoxia, and TGF-β signaling, as well as immunological features in the TME, including the estimated proportion of leukocytes, lymphocytes, regulatory T-cells, cytolytic activity, and PD-1 and PD-L1 expression were calculated for each cancer type, and the results are summarized in Figure 2.