Specifically, the objectives of this retrospective study were (1) to evaluate the pH- and oxygen-sensitive imaging features associated with glioma genotypes, including isocitrate dehydrogenase (IDH) mutation, 1p/19q co-deletion, and epidermal growth factor receptor (EGFR) amplification, (2) to assess the ability of the proposed imaging technique to differentiate different glioma genotypes, and (3) to investigate the association between these imaging biomarkers with patient overall survival (OS) and progression-free survival (PFS). The gene discussed is IDH3A; the disease is central nervous system cancer.