To investigate the effects of transient NEDD9 depletion on DDR machinery, we siRNA-depleted NEDD9 expression in the A549, H460, and H1299 NSCLC cell lines for 48 h, with an additional cisplatin treatment (500 nM, 6 h) benchmark, and then probed the expression and activity of the single- and double-stranded DNA damage response associated proteins using Western blotting. The gene discussed is NEDD9; the disease is non-small cell lung carcinoma.