Patient-derived GBM TSCs usually show strong expression of Nestin, Musashi-1, Sox-2, Oct-4, and BMI1, while differentiated cells express markers according to the cell type and degree of differentiation, such as GFAP (glial fibrillary acidic protein), Tuj-1, NeuN, beta-III-Tubulin, Olig-1. Here, GFAP is linked to glioblastoma.