In this study, we screened the deregulated signaling in the surgical tissues of CCA patients and found the activities and/or expression of several oncogenic molecules downstream of RTKs, including hydrogen peroxide clone5 (Hic-5), Src, AKT, and JNK known to be essential for metastasis, elevated in tissues of a significant portion of metastatic CCA. Here, AKT1 is linked to cholangiocarcinoma.