The samples were processed for Western blot of a lot of critical signal molecules known to be involved in tumor metastasis of CCA, including the oncogenic RTKs (EGFR, Her2, Her3, and c-Met) [10,11,12]; Src, one of the nonreceptor tyrosine kinase [13,14]; JNK, one of the MAPK [15,16]; and AKT, a downstream molecule of PI3K [17,18,19,20]. This evidence concerns the gene ERBB3 and cholangiocarcinoma.