Most likely, the stimulatory supportive effects of dietary soy on ASPH expression were due to preservation of signaling through IN-R and Akt rather than increased levels of peripheral blood insulin, since a follow-up analysis of serum insulin levels using a commercial magnetic bead-based Diabetes Bio-Plex Panel (Bio-Rad Laboratories, Hercules, CA, USA) showed similar (not statistically significantly different) levels across all four study groups (data not shown). Here, ASPH is linked to diabetes mellitus.