Hinokiflavone induced p53 activation was observed by (1) increased p53 phosphorylation at Ser15 in AML-2 and HCT116 cells treated with Hinokiflavone (Figure 4c,d) and (2) by higher mRNA expression of the p53 target genes p21 (CDKN1A), PUMA and MDM2 in HCT116 cells treated with Hinokiflavone, but not in HCT116 p53 null cells (Figure S5). This evidence concerns the gene CDKN1A and acute myeloid leukemia.