The up-regulated β3-AR plays a crucial role in lipid accumulation or storage in differentiating 3T3-L1 cells, mediated through modulation of expression and phosphorylation levels of C/EBP-α, PPAR-γ, STAT-5, FASN, and perilipin A. The present findings suggest that inhibitors of β3-AR expression or β3-AR antagonist(s) may be used as potential preventive and therapeutics against obesity. Here, ADRB3 is linked to obesity due to melanocortin 4 receptor deficiency.