It was found that the ACG in 95% ethanol extract of ZZX played an anti-anxiety role by regulating the prolactin signaling pathway, estrogen signaling pathway, and ARA metabolism pathway, as well as affecting important targets such as ALB, AKT1, PTGS2, CYP3A4, ESR1, CASP3, CYP2B6, EGFR, SRC, MMP9, IGF1, and MAPK8. This evidence concerns the gene ESR1 and Anxiety.