Using ENCODE and BLUEPRINT, enrichment of H3K27ac at the E2 enhancer region of MEIS1 was further validated in MEIS1-high primary human CD34+ cells as well as primary MLL-AF9 AML patients samples compared to MEIS1-low PBMCs and inv [16] respectively (Supplementary Fig. S7a, b). This evidence concerns the gene CD34 and acute myeloid leukemia.