Additionally, the ratio of CD8+ T cells to regulatory T Cells (Tregs) correlates with positive clinical outcome,29 and TGFβ in tumor promotes cancer progression and represses the antitumor immunity.30 In our analysis, the ratio of CD8+ T /Tregs and the expression of TGFβ showed significant increase and decrease respectively in I.T. group when compared with control and S.C. group (online supplemental figure S6D, E). The gene discussed is TGFB1; the disease is cancer.