TGFB1 and neoplasm: Additionally, the ratio of CD8+ T cells to regulatory T Cells (Tregs) correlates with positive clinical outcome,29 and TGFβ in tumor promotes cancer progression and represses the antitumor immunity.30 In our analysis, the ratio of CD8+ T /Tregs and the expression of TGFβ showed significant increase and decrease respectively in I.T. group when compared with control and S.C. group (online supplemental figure S6D, E).