In this study, we demonstrated that intratumoral injection maintained LN targeting of nanovaccines to generate tumor-specific T cells and induced intratumoral CXCL9 expression to initiate a myeloid cell/CXCL9-CD8+ T cell/IFNγ feedback loop for CD8+ T cell infiltration, leading to a stronger antitumor effect and long-term immune memory than conventional subcutaneous immunization. This evidence concerns the gene IFNG and neoplasm.