For example, circANKS1B increases the expression of the transcription factor USF1 by sponging miR-148a-3p and miR-152-3P, which in turn upregulates TGF-β1, activates the TGF-β1/Smad signalling pathway, promotes the conversion of breast epithelial cells into mesenchymal cells, and finally promotes breast cancer invasion and metastasis [11]; Cir-GLI2 promotes proliferation and migration as well as the invasion of osteosarcoma cells by targeting miR-125b-5p [12]. The gene discussed is TGFB1; the disease is breast carcinoma.