Cytotoxicity of the samples was tested using several cell lines, including HDF, human breast adenocarcinoma (T47D), African green monkey kidney (Vero E6), human large intestine adenocarcinoma (HCT-8), and human lung epithelial cell lines overexpressing ACE2 and TMPRSS2 (A549ACE2/TMPRSS2). The gene discussed is TMPRSS2; the disease is colorectal adenocarcinoma.