Therefore, this present study acquired the list of the HTLV-1 associated genes and co-DEGs in HCC and then identified MAD2L1, CCNB2, CDC20, PTTG1, BUB1B, FOS, and EGR1, as the seven HTLV-1 infection-related genes which were significantly and differentially expressed in HCC tissues through Venn diagram screening. This evidence concerns the gene PTTG1 and hepatocellular carcinoma.