Our data revealed differential phosphorylation of proteins such as HSP90AA1, ACLY, DNAJC5, HRNR, RAB31, S100A8, and S100A9 associated with degranulation process in response to Hcy and HG which may lead to altered and excessive neutrophil degranulation, subsequently triggering vascular damage in diseases such as T2D and atherosclerosis. Here, S100A8 is linked to type 2 diabetes mellitus.