Figure 8A lists the top 50 transcription factors according to the t values. Among them, GABPA, CHD2, KDM5A, MAX, and SP1 were considered as the main transcription factors. Compared to controls, GABPA, CHD2, and KDM5A had higher AUC values.MAX and SP1 had lowered AUC values in diabetic nephropathy (Figure 8B). Immune cells had the high regulation activity of CHD2, MAX, SP1, KDM5A, and GABPA based on scRNA-seq data (Figure 8C). This evidence concerns the gene SP1 and diabetic kidney disease.