Figure 2G shows that AQP5 protein levels were overexpressed compared to immunohistochemistry in clinical PAAD tissue. Similarly, we confirmed this in the PAAD cell lines. AQP5 mRNA and protein levels were overexpressed compared with real-time PCR, Western blotting, and immunofluorescence (Figures 2H–J). The gene discussed is AQP5; the disease is pancreatic adenocarcinoma.