To define the expression profiles of circRNA in PIK3CA-mutant CRC cells, circRNA sequencing by Hiseq, which captured 78,346 circRNAs, was implemented using HCT116 cells with PIK3CAWT or PIK3CAH1047R as well as DLD1 cells with PIK3CAWT or PIK3CAE545K (step1 as shown in Fig. 1B). Here, PIK3CA is linked to colorectal carcinoma.