According to the disease annotation in KOBAS database, 22 CHD-related DE mRNAs were identified, in which the proteins encoded by DNA damage inducible transcript 3 (DDIT3), sal-like protein 4 (SALL4), nuclear receptor interacting protein 1 (NRIP1), ankyrin repeat domain 6 and estrogen receptor alpha gene (ESR1) were transcription factors (TFs) (Additional file 1: Table S1). The gene discussed is ESR1; the disease is coronary artery disorder.