PTCH1 and neoplasm: In order to establish the Hh signaling-related tumor model, we disrupted the wild-type PTCH1 allele remaining in three NBCCS-derived iPSC lines, G11-iPSC, G36-iPSC, and G72-iPSC [21, 22], by introducing the CRISPR/Cas9 expression vector, which targets the wild-type PTCH1 sequence.