These ASOs significantly reduced their respective 3′ SP expression with a concomitant increase in the unspliced, full-length transcript (3′ FL) for CHEK1, CTNNB1 and THUMPD1, while the CDS transcripts were unaffected in the HCC cell lines, Hep3B and HepG2 (Fig. 3e,f, Extended Data Fig. 3c,d and Supplementary Note). The gene discussed is CHEK1; the disease is hepatocellular carcinoma.