To test whether these juxta-tumoral myofibroblasts were directly attached to tumor cells, pancreases from Pdx1-Cre; LSL-KrasG12D/+; Trp53flox/flox (PdKP53) mice and control littermates (Pdx1-Cre; LSL-Kras+/+; Trp53flox/flox (WT)) between 6 to 7 weeks old were collected and mechanically dissociated to cell clusters with a gentle MACSTM dissociator (Milteny Biotec) (Fig. 2a). Here, PDX1 is linked to neoplasm.