MYOD1 and Menkes disease: To investigate the mechanism whereby MyoD expression induces or worsens MD pathology, we generated a C2C12 myotube cell culture model with doxycycline-inducible MyoD expression from a recombinant lentivirus, followed by mRNA expression profiling (with or without MyoD overexpression (+Dox)) (Supplementary Fig 5b).