Indeed, direct analysis of mRNA expression in the TA muscle from either AAV-MyoD or AAV-Mist1 injected mice showed significant regulation of the membrane fusion effectors, myomaker and myomerger (Supplementary Fig 5c, d), which is especially relevant given that specific deletion of myomaker in myofibers was recently shown to reduce muscular dystrophy pathological features in the mouse19 (see discussion below). The gene discussed is BHLHA15; the disease is muscular dystrophy.