Remarkably, even Wt mice subjected to AAV-MyoD protein induction from 4 to 6.5 months (Fig. 4g, h) exhibited MD related pathology (i.e., central nucleation, sarcolemmal permeability to IgM, fibrosis, and increased susceptibility to eccentric contraction injury) compared with control AAV infection (Fig. 4i–m). This evidence concerns the gene CD40LG and Menkes disease.