Western blot analysis showed that compared with the NC group, the protein levels of NLRP3, ASC, Cleaved caspase-1 and Cleaved IL-1β were significantly increased in the DM group, while BBR treatment reduced the expression of NLRP3, ASC, Cleaved caspase-1 and Cleaved IL-1β (Figure 3B, p < 0.05). The gene discussed is IL1B; the disease is diabetes mellitus.