The effect of functional AMS induction was then tested using a DEX-inducible AMS construct (AMSprom:AMS-GR-YFP from Ferguson et al., 2017), which 24 h after DEX treatment resulted in AMS protein localized to the nucleus and a reduction in the expression of all the putative targets when compared with AMS–GR–YFP prior to DEX treatment (normalized to 1 to aid comparison), with TPR-like showing the strongest association (Fig. 7D). Here, NR3C1 is linked to ablepharon macrostomia syndrome.