The body mass index, endotoxin activity, and concentrations of aspartate aminotransferase, alanine amino transferase, gamma‐glutamyl transpeptidase, total cholesterol, triglycerides, low-density lipoproteins, hyaluronic acid, and type IV collagen 7s domain were significantly higher and those of platelets, albumin, and high‐density lipoproteins were significantly lower in patients with severe fibrosis NAFLD than in HCs. This evidence concerns the gene GGTLC1 and metabolic dysfunction-associated steatotic liver disease.