We did not use HCC adjacent normal tissue as HCC usually occurs in the context of ongoing liver disease and we have published that SRSF3 is lost in early liver disease.[4a] The HCC samples showed high levels of IGF2 protein compared to normal liver by immunoblotting (Figure 7e,f) consistent with other published data.[9g] Immunohistochemical staining of fixed liver sections confirmed that the IGF2 expression was in hepatocytes (Figure 7g). Here, SRSF3 is linked to hepatocellular carcinoma.