This mechanism is related to increased acetyl cholinesterase (AChE) content and reduced choline acetyltransferase (ChAT) expression (Wang et al., 2014), suggesting that with the development of the biological-psychological-social medical model, Qi depression caused by an accumulation of negative emotions has been proven to be closely related to the progression of cognitive decline (Zhou et al., 2006; Ismail et al., 2018; Zhang et al., 2021). The gene discussed is CHAT; the disease is major depressive disorder.