The expressions of miR-25-3p and MAP2K4 in LC tissues and ANT of 74 LC patients were detected by qRT-PCR, and the data demonstrated that miR-25-3p level was upregulated and MAP2K4 level was downregulated in the LC tissues (P < 0.001, Figures 6(a) and 6(b)). The gene discussed is MAP2K4; the disease is laryngotracheoesophageal cleft.