ACTB and melanoma: We constructed a rAAV vector containing the ATM promoter (Figure 3C) and compared its expression intensity with the broad-spectrum CB (chicken β-actin promoter plus cytomegalovirus enhancer) promoter in immortalized 293T cells, the human esophageal squamous carcinoma cell line SBRC-EC01, and the mouse melanoma cell line B16 through plasmid transfection.