Then, we used N-acetylcysteine (NAC), a classical ROS scavenger, to block ROS elevation and found that NAC prevented AD-induced the generation of ROS (Figure 3B) and markedly attenuated AD-inhibited ER-α expression both at protein (Figures 3C, D) and mRNA levels (Figures 3E, F). The gene discussed is ESR1; the disease is Alzheimer disease.