More importantly, we found that loss of Trp53 and Rb1 in Ctsk+ cells promoted osteosarcoma cell migration and invasion; however, ablation of YAP signaling delayed osteosarcoma progression in Ctsk‐Cre;Trp53f/f/Rb1f/f mice due to the inhibition of migration and invasion. Here, RB1 is linked to osteosarcoma.