The KEGG analysis revealed that the differential genes were primarily enriched in “Phagosome,” “Focal adhesion,” “Staphylococcus aureus infection,” “GABAergic synapse,” “Complement and coagulation cascades Leishmaniasis,” “Viral myocarditis,” “Amoebiasis,” “Nicotine addiction,” “ECM-receptor interaction,” “AGE-RAGE signaling pathway in diabetic complications Type I diabetes mellitus,” “Morphine addiction,” “Glutamatergic synapse,” “Synaptic vesicle cycle,” “Type I diabetes mellitus,” “Insulin secretion,” “Retrograde endocannabinoid signaling,” “Neuroactive ligand-receptor interaction”. This evidence concerns the gene INS and nicotine dependence.