In addition, the results of qRT-PCR and Western blot demonstrated that versus HET-1A cells, the expression of SOX17 was reduced in ESCC cell lines (KYSE70, KYSE170, KYSE150, and KYSE510), with KYSE150 cells presenting the lowest SOX17 expression (Fig. 1D, E) and thus selected for the experiments related to SOX17. This evidence concerns the gene SOX17 and esophageal squamous cell carcinoma.