Here, we used two-colour single-molecule total internal reflection fluorescence (TIRF) microscopy for super-resolved single-molecule localization microscopy (SMLM) on live human colon carcinoma cells stably expressing EGFR–GFP in the presence of tetramethylrhodamine (TMR) conjugated to EGF (figure 1). This evidence concerns the gene EGFR and colon carcinoma.