Taken together, our data using the ALTEN platform demonstrated that IL2 primarily induced CD8 T cells into cytotoxic cells in the tumor microenvironment at 24 h using the ALTEN platform, recapitulating the effects of IL2 observed in vivo recently.[18] These results suggest that ALTEN provides an excellent novel platform for ex vivo immunomodulation studies. The gene discussed is CD8A; the disease is neoplasm.