In order to simulate the effect of infection status on the NLRP3 expression level and cell pyrolysis, we stimulated different cell lines (HEK cells, HEK293 and Human Kidney-2, HK2) with varying concentrations of LPS (PBS, 0.1 μg/ml, 1 μg/ml, and 10 μg/ml) for different time (0 h, 6 h, 12 h, and 24 h), and detected the NLRP3 transcription level by qPCR. Here, NLRP3 is linked to infection.