Considering the natural history of NAFL and NASH, the major conditions are as follows: NAFL pattern 8 all negative, NASH hepatitis pattern 3 (ALT(+), IV collagen(−), E(+)), NASH fibrosis pattern 1 (ALT(+), IV collagen(+), E (+) all positive), and NASH LC pattern 5 (ALT(−), IV collagen(+), E (+)). Here, GPT is linked to non-alcoholic fatty liver.