From the result of the ROC curve (Figure 2), cutoff value (1) was set to 44 IU/L of ALT for distinguishing between NAFL and NASH s0-1/NASH s2-3, whereas cutoff value (2) was set to 36 IU/L for distinguishing between NASH s0-1/NASH s2-3 and NASH s4/LC. This evidence concerns the gene GPT and non-alcoholic fatty liver.