We chose the top 50 hub genes to enter into GEPIA to screen (Fig. 9 and Fig. S4), and the 150 hub genes in the upregulated circRNA-downregulated miRNA-upregulated mRNA (UcDiUm-RNA) network, there was higher expression for 12 hub genes (CCL4, APLN, FBXO22, SH3KBP1, CKAP4, PDIA6, RCN1, TP53, CCND1, EZH2, E2F2, CASP3) in the CRC group as compared to normal tissues group. The gene discussed is E2F2; the disease is colorectal carcinoma.