Furthermore, we clarified that the interaction between TROY and p85α could lead to p85α degradation and subsequently activate PI3K/AKT/TBX3 signaling, which helps to maintain the pluripotency of liver CSCs by upregulating the expression of SOX2, NANOG, and OCT4 and promote cell motility via activating EMT pathway in HCC. The gene discussed is SOX2; the disease is hepatocellular carcinoma.