It came as no surprise that the ROC curve analysis also confirmed the fairly high sensitivity and specificity of these combined six proteins for distinguishing ESCC patients from controls (sensitivity: 0.906 and specificity: 0.967, respectively, with an AUC of 0.945), which is clearly greater than that of S100A8/A9 (AUC: 0.887), SAA1 (AUC: 0.786), ENO1 (AUC: 0.817), TPI1 (AUC: 0.746) and PGAM1 (AUC: 0.681) alone. The gene discussed is S100A8; the disease is esophageal squamous cell carcinoma.