In this study, we observed concomitant genes with ex20ins significantly enriched in PI3K/AKT, microRNAs in cancer, and Rap1 signaling pathways by KEGG analysis, revealing the upregulation of PI3K/AKT signaling was much more common and important in NSCLC with EGFR ex20ins, which had been reported in HER2‐positive breast cancer.36 This evidence concerns the gene PIK3CA and cancer.