Flow cytometric analysis of cell cycle progression showed that silencing of GK‐IT1 remarkably increased ESCC cell distribution in the G1 phase and reduced distribution in the S phase, which suggests that cell cycle arrest in the G1 phase was induced by knockdown of GK‐IT1 (Figure 4A and Figure S3A,B). The gene discussed is GK-IT1; the disease is esophageal squamous cell carcinoma.