In agreement with Kim et al. [31] and Moon et al. [47], we have restored the expression of EYA4 after treating the Caco-2 and LoVo colon cancer cells with 1 μM 5-Aza-CdR for 72 hours, this implies the possible mechanism of involvement of DNA methylation in hampering the expression of EYA4 [31], which could accelerate colon cancer formation [31]. Here, EYA4 is linked to malignant colon neoplasm.