Colon cancer cell lines LoVo and Caco-2 were treated with 1 μM 5-Aza-CdR for 72 hours, and the expression of EYA4 was significantly upregulated in LoVo (Figure 4(a)) and Caco-2 (Figure 4(b)) compared to cells treated with DMSO. The gene discussed is EYA4; the disease is colonic neoplasm.