IFNG and neoplasm: After stimulation of lymphocytes with autologous tumor cells, we identified tumor-reactive T cells in lymphocytes isolated from non-metastatic lymph nodes of C126 by IFNγ ELISPOT assay, but we found tumor-reactive T cells in lymphocytes isolated from metastatic lymph nodes in two remaining cases (Additional file 1: Fig. S1a and Table 2).