Histological examination revealed that HCT116EHF + CDX1 tumours were more differentiated, evidenced by increased gland formation and expression of the differentiation markers VIL1 and KRT20, compared to HCT116EV, HCT116EHF or HCT116CDX1 tumours (Fig. 5J, S5A). This evidence concerns the gene VIL1 and neoplasm.