ISX and colorectal carcinoma: While simultaneous knockdown of EHF with GATA6, ISX or ELF3 did not alter expression of differentiation markers (Fig. S3), combined EHF/CDX1 knockdown markedly repressed expression of multiple differentiation markers in SW948 cells (Fig. 2A, B), findings that were confirmed in a second moderately-differentiated CRC cell line, SW403 (Fig. S4A, B).