AKT1 and cancer: Interestingly, although inhibition of CD59 leads to a significant increase in the susceptibility of CaSki-NANOG cells to NHS-mediated CDC, CD59 did not influence the hyperactivation of AKT signaling, which is essential for immune resistance to CTL killing as well as stem-like property of NANOG+ tumor cells13 and the cancer stem cell (CSC)-like property (Supplementary Fig. S5).