To further evaluate the effect of M2-EVs on the osteogenesis of AS-BMSCs, the expression of osteogenic differentiation markers Runx2 and osteocalcin (OCN) was detected by reverse-transcription quantitative polymerase-chain reaction (RT-qPCR) and Western blot analysis in AS-BMSCs after coculture in OM containing M2-EVs at different concentrations for 7 days. This evidence concerns the gene BGLAP and ocular melanoma.