To confirm the resistance to CYH33 in ESCC mediated by EZH2, we generated KYSE510 cells stably expressing EZH2 by transfecting dCas‐VP64, MS2‐p65‐HSF1 and EZH2‐targeted sgRNA included in the CRISPR‐SAM library. Here, HSF1 is linked to esophageal squamous cell carcinoma.