To do this, PCa cells were treated with buthionine sulphoximine (BSO), an inhibitor of gamma-glutamylcysteine synthetase (gamma-GCS) which lowers cellular GSH concentrations[42] for 24 h prior to the addition of Nox2wt and Nox2−/− neutrophils. Here, GCLC is linked to posterior cortical atrophy.